spectroscopy-py

Spectroscopy - Console mode

Table of Contents

1. Console Usage
   • Sync in frequency
   • Start the data acquisition
2. Useful links
3. License
4. Contact

Console usage

The Spectroscopy Console mode provides live-streaming data representation directly in the console, without an interface intermediary and charts data visualization processes. The data displayed on the console screen indicates the channel (number), the moment of acquisition (in seconds) and the corresponding decay curve values of 256 chart points detected during that time.

Here a table summary of the configurable parameters:

	data-type	config	default	explanation
sync_in_frequency_mhz	number	set the value manually or set the function detect_laser_sync_in_frequency() to use automatic laser frequency detection	check the Sync in frequency paragraph for more detailed information.
selected_sync	string	choose a value from "sync_in", "sync_out_10", "sync_out_20", "sync_out_40", "sync_out_80"	"sync_in"	select whether you will connect to the sync_in or the sync_out port of your FLIM LABS acquisition card
enabled_channels	number[]	set a list of enabled acquisition data channels (up to 8). e.g. [0,1,2,3,4,5,6,7]	[1]	the list of enabled channels for photons data acquisition
bin_width_micros	number	Set the numerical value in microseconds	1000 (ms)	the time duration to wait for photons count accumulation.
acquisition_time_millis	number/None	Set the data acquisition duration	None	The acquisition duration could be determinate (numeric value) or indeterminate (None)

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Sync in frequency

Perform this step only if selected_sync="sync_in".

Set the value of the sync_in_frequency_mhz variable.
The value can be set manually by modifyng the line sync_in_frequency_mhz=0.0.

Alternatively, the automatic laser frequency detection feature, can be used by commenting out the line sync_in_frequency_mhz=0.0 and uncommenting the line # sync_in_frequency_mhz= detect_laser_sync_in_frequency() .

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Start the data acquisition

Once the variables settings is completed, the software is ready to start the data acquisition. To run the process the console should be opened inside the project directory /SPECTROSCOPY-PY and receive the following commands:

• python -3.9 -m venv venv
• venv/scripts/activate
• pip install -r requirements.txt
• python console.py

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Useful Links

For more details about the project follow these links:

• Spectroscopy introduction
• Spectroscopy GUI guide

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License

Distributed under the MIT License.

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Contact

FLIM LABS: info@flimlabs.com

Project Link: FLIM LABS - Spectroscopy

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